%0 Journal Article
%T Rabies virus glycoprotein variants display different patterns in rabies monosynaptic tracing
%A Takuma Mori
%J Frontiers in Neuroanatomy
%D 2014
%I Frontiers Media
%R 10.3389/fnana.2013.00047
%X Rabies virus (RV) has been widely used to trace multi-synaptic neuronal circuits. The recent development of glycoprotein-deficient rabies virus (RV-ĶĪG) expressing various proteins has enabled analyzes of both the structure and function of neuronal circuits. The main advantage of RV-ĶĪG is its ability to trace monosynaptic circuits by the complementation of rabies virus glycoprotein (RVG), but it has the disadvantage of cytotoxicity. Several strain variants of RV have different biological characteristics, such as synaptic spreading and cytotoxicity, mainly due to amino acid mutations in RVG. We developed an improved protocol for the production of a highly attenuated strain of RV-ĶĪG and assessed whether RVG variants affect rabies monosynaptic tracing and the health of infected neurons. We demonstrated that (1) rabies monosynaptic tracing with RVG variants traced different subsets of presynaptic partners, (2) RVG of the attenuated strain also labeled astrocytes, and (3) the cytotoxicity of RV-ĶĪG did not depend on RVG but on RV-ĶĪG. These findings indicate that RVG variants are an important determinant of rabies monosynaptic tracing.
%K trans-synaptic tracing
%K neuronal degeneration
%K neocortex
%K in utero electroporation
%K astrocytes
%U http://www.frontiersin.org/Journal/10.3389/fnana.2013.00047/abstract