%0 Journal Article
%T JcTI-I, a novel trypsin inhibitor from Jatropha curcas seed cake with potential for bacterial infection treatment
%A Helen Paula S. Costa
%A Jose Tadeu A. Oliveira
%A Daniele O. Sousa
%A Janne Keila S. Morais
%A Frederico B. Moreno
%A Ana Cristina O. Monteiro-Moreira
%A Ricardo A. Viegas
%A Ilka M. Vasconcelos
%J Frontiers in Microbiology
%D 2014
%I Frontiers Media
%R 10.3389/fmicb.2014.00005
%X Jatropha curcas seed cake is a low-value by-product resulting from biodiesel production. The seed cake is highly toxic, but it has great potential for biotechnology applications as it is a repository of biomolecules that could be important in agriculture, medicine and industry. To explore this potential, a novel trypsin inhibitor called JcTI-I was purified by fractionation of the crude extract with trichloroacetic acid (2.5%, v/v) followed by affinity chromatography (Trypsin-Sepharose 4B) and molecular exclusion (Sephacryl S-200). Non-reducing SDS-PAGE and gel filtration showed that JcTI-I has approximately 20.0 kDa. Mass spectrometry analysis revealed that the intact molecular mass of JcTI-I is 10.252 kDa. Moreover, JcTI-I is a glycoprotein with 6.4% (m/m) carbohydrates, pI of 6.6, N-terminal sequence similarity around 60% to plant albumins and high stability to heat, pH and salinity. JcTI-I presented antibacterial activity against the human pathogenic bacteria Salmonella enterica subspecies enterica serovar choleraesuis and Staphylococcus aureus, with minimum inhibitory concentration (MIC) less than 5 ¦Ìg/mL. Furthermore, JcTI-I did have inhibitory activity against the serine proteases from the tested bacteria. Otherwise, no hemolytic activity of human erythrocytes and signs of acute toxicity to mice were observed for JcTI-I. The results demonstrate the benefits of J. curcas seed cake as a source of trypsin inhibitor with potential for biotechnological application as a new antimicrobial agent against human pathogenic bacteria.
%K Jatropha curcas
%K seed cake
%K serine proteinase inhibitor
%K trypsin inhibitor
%K Bacterial Infections
%K antimicrobial agent
%U http://www.frontiersin.org/Journal/10.3389/fmicb.2014.00005/abstract