%0 Journal Article
%T Cytoplasmic 5¡¯-3¡¯ exonuclease Xrn1p is also a genome-wide transcription factor in yeast
%A Daniel A. Medina
%A Antonio Jord¨¢n-Pla
%A Gonzalo Mill¨¢n-Zambrano
%A Sebasti¨¢n Ch¨¢vez
%A Mordechai Choder
%A Jos¨¦ E. P¨¦rez-Ort¨ªn
%J Frontiers in Genetics
%D 2014
%I Frontiers Media
%R 10.3389/fgene.2014.00001
%X The 5¡¯ to 3¡¯ exoribonuclease Xrn1 is a large protein involved in cytoplasmatic mRNA degradation as a critical component of the major decaysome. Its deletion in the yeast Saccharomyces cerevisiae is not lethal, but it has multiple physiological effects. In a previous study, our group showed that deletion of all tested components of the yeast major decaysome, including XRN1, results in a decrease in the synthetic rate and an increase in half-life of most mRNAs in a compensatory manner. Furthermore, the same study showed that the all tested decaysome components are also nuclear proteins that bind to the 5¡¯ region of a number of genes. In the present work, we show that disruption of Xrn1 activity preferentially affects both the synthesis and decay of a distinct subpopulation of mRNAs. The most affected mRNAs are the transcripts of the highly transcribed genes, mainly those encoding ribosome biogenesis and translation factors. Previously, we proposed that synthegradases play a key role in regulating both mRNA synthesis and degradation. Evidently, Xrn1 functions as a synthegradase, whose selectivity might help coordinating the expression of the protein synthetic machinery. We propose to name the most affected genes ¡°Xrn1 synthegradon¡±.
%K Transcription rate
%K nascent transcription
%K mRNA synthesis
%K mRNA decay
%K mRNA stability.
%U http://www.frontiersin.org/Journal/10.3389/fgene.2014.00001/abstract