%0 Journal Article %T Methodology Article: Can Ruminal Reducing Power Assessed in Batch Cultures be Comparable to <i>in Vivo</i> Measurements? %A Christine Julien %A Jean-Philippe Marden %A Annabelle Troegeler-Meynadier %A Corine Bayourthe %J Journal of Analytical Sciences, Methods and Instrumentation %P 80-86 %@ 2164-2753 %D 2014 %I Scientific Research Publishing %R 10.4236/jasmi.2014.43011 %X
In ruminant field of digestive research, the appeal to methods of less invasive studies and reproducing the in vivo conditions is essential. The objective of the present study was to determine whether the conditions created with the proposed in vitro batch culture was an accurate reproduction of the physico-chemical and fermentative ruminal conditions observed in vivo. Two experiments were conducted to compare ruminal reducing power measured in vitro, i.e. in batch cultures or, in vivo i.e. in live animals: dairy cows at maintenance (Experiment 1) and lactating dairy cows (Experiment 2). In Experiment 1, at the beginning of incubation period, in vitro redox potential (Eh), pH and ClarkĄ¯s exponent (rH) values were significantly higher than in vivo (+42 mV, +0.25 and +1.9, respectively) whereas volatile fatty acids (VFA) %K Batch Cultures %K ClarkĄ¯s Exponent %K Redox Potential %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=49424