%0 Journal Article
%T Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry
%A Olga S. Brushinina
%A Roman V. Gurto
%A Maksim S. Timofeev
%A Galina A. Frelikh
%A Vladimir A. Slepichev
%A Elena A. Yanovskaya
%A Natalia U. Polomeeva
%A Yulya G. Zyuz¡¯kova
%A Vladimir V. Udut
%J International Journal of Analytical Mass Spectrometry and Chromatography
%P 25-32
%@ 2332-1776
%D 2014
%I Scientific Research Publishing
%R 10.4236/ijamsc.2014.22003
%X <p align=\"justify\">
	Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatograph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 ¦Ìm 250 ¡Á 3.0 mm with a pre-column cartridge PerfectBond ODS-HD 5 ¦Ìm 10 ¡Á 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng/ml. m/z for CDS 441.20¡ªpositive scan, m/z for HCTZ 295.90¡ªnegative scan. The method has been applied to a pharmacokinetic study of 12.5 mg HCTZ and 16 mg CDS tablet in healthy volunteers.
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%K Candesartan
%K Hydrochlorothiazide
%K LC-MS
%K Pharmacokinetic
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47497