%0 Journal Article
%T Dodecylamine Derivative of  Hydroxocobalamin Acts as a Potent  Inhibitor of Cobalamin-Dependent  Methionine Synthase in Mammalian  Cultured COS-7 Cells
%A Tomohiro Bito
%A Mariko Yasui
%A Toshio Iwaki
%A Yukinori Yabuta
%A Tsuyoshi Ichiyanagi
%A Ryoichi Yamaji
%A Yoshihisa Nakano
%A Hiroshi Inui
%A Fumio Watanabe
%J Food and Nutrition Sciences
%P 1318-1325
%@ 2157-9458
%D 2014
%I Scientific Research Publishing
%R 10.4236/fns.2014.514143
%X <a></a><a></a>We
evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a
potent inhibitor of cobalamin-dependent enzymes in an African green monkey
kidney cell, COS-7. When the dodecylamine derivative (1.0 ¦̀mol/L) did not show
any cytotoxicity in the cultured cells, the derivative could not affect
methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit
methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7
grown in 1.0 ¦̀mol/L hydroxocobalamin-supplemented medium. An immunoblot
analysis indicated that the dodecylamine derivative could not decrease the
protein level of methionine synthase, but significantly inhibit the enzyme
activity.
%K Dodecylamine Derivative
%K Cobalamin
%K COS-7 Cell
%K Enzyme Inhibitor
%K Hydroxocobalamin
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=48538