%0 Journal Article
%T Antioxidant Effects of Alperujo Extract (Arbequina and Frantoio Varieties) on MIN6 <i>¦Â</i>-Cells Subjected to Stress with Glucose or H<sub>2</sub>O<sub>2</sub>
%A C¨¦sar Gonz¨¢lez
%A M¨®nica Andrews
%A Elba Leiva
%A Cristina Quispe
%A Miguel Arredondo
%J Food and Nutrition Sciences
%P 1280-1289
%@ 2157-9458
%D 2014
%I Scientific Research Publishing
%R 10.4236/fns.2014.513139
%X <p>
	Alperujo, an
antioxidant-rich by-product of olive oil extraction, could protect <em>¦Â</em>-cells against oxidative damage. Our
goal was to study the antioxidant effects of an alperujo extract (AE) on MIN6 <em>¦Â</em>-cells challenged with glucose or
hydrogen peroxide. MIN6 <em>¦Â</em>-cells were
challenged with glucose (100 mM) or H<sub>2</sub>O<sub>2 </sub>(0.15 mM), with
or without AE (20 ¦ÌM phenol). Reactive oxygen species, intracellular iron (Fe),
insulin, glucose uptake, and mRNA gene expression of Uncoupling Protein-2
(UCP-2), Thioredoxin (TRDX), p47phox, and the ratio Bax/Bcl-2 were measured.
ROS increased when the stressors were incubated with AE (p &lt; 0.05 and p &lt;
0.01, respectively). Intracellular Fe increased in glucose presence (100 mM p &lt;
0.001). Insulin secretion improved when cells were pre-incubated with AE (p &lt;
0.001) and glucose uptake increased when cells were pre-incubated with AE for 3
days and then further treated with glucose (p &lt; 0.001). After 3 days of AE
alone, mRNA relative expression of UCP-2 and TRDX increased (p &lt; 0.001) and
after 5 days p47phox, also increased. The Bax/Bcl-2 ratio tended to decrease in
the samples pre-incubated with AE. The Alperujo extract,in vitro, had a pro-oxidant behavior, however pre-incubating MIN6 <em>¦Â</em>-cells with AE tended to protect them
against apoptosis, thereby enhancing insulin secretion.
</p>
%K Alperujo
%K Oxidative Stress
%K Apoptosis
%K Glucose
%K &lt
%K i&gt
%K ¦Â&lt
%K /i&gt
%K -Cells
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=48217