%0 Journal Article
%T Proteomic Analysis of Celecoxib on  Chondrocytes from Patients with Osteoarthritis
%A Kenji Takenouchi
%A Mitusmi Arito
%A Toshiyuki Sato
%A Kenji Takahashi
%A Manae S. Kurokawa
%A Kazuo Yudoh
%A Shinro Takai
%A Tomohiro Kato
%A Hiroshi Nakamura
%J Modern Research in Inflammation
%P 90-98
%@ 2169-9690
%D 2014
%I Scientific Research Publishing
%R 10.4236/mri.2014.33011
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:12px;font-family:Verdana;\">Objective: To study
a comprehensive proteomic analysis of celecoxib in oseteoarthritis (OA) chondrocytes.
Methods: OA chondrocytes were stimulated with celecoxib, IL-1</span><i><span style=\"font-size:12px;font-family:Verdana;\">¦Â</span></i><span style=\"font-size:12px;font-family:Verdana;\"> and IL-1</span><i><span style=\"font-size:12px;font-family:Verdana;\">¦Â</span></i><span style=\"font-size:12px;font-family:Verdana;\"> together with
celecoxib. Proteins were extracted from the cells and subjected to
2-dimensional differential image gel electrophoresis (2D-DIGE). Proteins of
interest were identified by mass spectrometry. Results: Eighty-six protein
spots showed significantly different intensities with each reagent or reagent
combination. AAA+ protein, HSP47/Serpin, cAMP-dependent protein kinase type
II-beta regulatory subunit, alpha-actin-4 and tubulin decreased with the
addition of celecoxib, while apolipoprotein A-V, glutamate carboxipeptide 2,
mitochondrial stress-70 protein, sorting nexin-9 and GRP78 increased with the
addition of celecoxib. GRP78 is a stress protein and may be chondroprotective.
Celecoxib modulated IL-1</span><i><span style=\"font-size:12px;font-family:Verdana;\">¦Â </span></i><span style=\"font-size:12px;font-family:Verdana;\">stimulated
chondrocytes, and CD200R and moesin were identified as such resulting proteins.
Conclusion: Protein profiles of OA chondrocytes changed after administration of
celecoxib. Further investigation is needed to elucidate the function of each
protein in OA chondrocytes.</span> 
</p>
%K Proteomic Analysis
%K Celecoxib
%K Chondrocytes
%K Osteoarthritis
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=48223