%0 Journal Article
%T The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum
%A Elham O. Mahgoub
%J Open Journal of Genetics
%P 316-331
%@ 2162-4461
%D 2014
%I Scientific Research Publishing
%R 10.4236/ojgen.2014.44029
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:10.0pt;font-family:;\" \"=\"\"><span style=\"font-family:Verdana;\">Chicken anaemia
virus (CAV) causes a viral disease in chickens worldwide and thus has economic
importance. The main aim of this study was to develop a rapid, sensitive and
specific VP1-CAVI indirect ELISA for the detection of CAV infection. The CAV-VP1,
was separately cloned and expressed in recombinant </span><i><span style=\"font-family:Verdana;\">E. coli</span></i><span style=\"font-family:Verdana;\">. The purified
recombinant CAV-VP1 protein was then coated as an antigen on an ELISA plates to
evaluate its reactivity against chicken sera. The resulting indirect ELISA was
then compared with a commercial ELISA. The specificity and sensitivity of the
indirect ELISA were measured as 93.3% and 100%, respectively. A t</span><i><span style=\"font-family:Verdana;\">-</span></i><span style=\"font-family:Verdana;\">test produced a t-value of 15.805 for the indirect
ELISA and revealed a significant difference</span><sup> </sup><span style=\"font-family:Verdana;\">between CAV-positive
serum and CAV-negative serum (p-value of 0.001). For the second variable (</span><i><span style=\"font-family:Verdana;\">i.e</span></i><span style=\"font-family:Verdana;\">., a commercial ELISA), the t-test
yielded a t-value of 5.063, which revealed a significant difference</span><sup> </sup><span style=\"font-family:Verdana;\">between
CAV-positive serum and CAV-negative serum (p-value of 0.015). This intervention
produces statistically significant improvements in both variables (p-values &lt; 0.05). The correlation
coefficient for the indirect ELISA was </span><i><span style=\"font-family:Verdana;\">r</span></i><span style=\"font-family:Verdana;\"> = 0.93. Therefore, this work
can be considered as a new achievement in diagnosis for Chicken anaemia virus
in chicken flocks.</span></span> 
</p>
%K Indirect ELISA
%K Commercial ELISA
%K Chicken Anaemia Virus
%K Recombinant Protein VP1
%K Sensitivity
%K Specificity
%K Standard Curve for Indirect ELISA
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=48052