%0 Journal Article
%T Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery
%A Rosa Mar¨ªa L¨®pez-Franco
%A Jorge E. Moreno-Cuevas
%A Mar¨ªa Teresa Gonz¨¢lez-Garza
%J Stem Cell Discovery
%P 61-66
%@ 2161-6787
%D 2014
%I Scientific Research Publishing
%R 10.4236/scd.2014.43007
%X <p class=\"MsoNormal\" style=\"text-align:justify;\">
	<span lang=\"EN-US\" style=\"font-size:12px;font-family:Verdana;\">Circulating CD133+ stem
cells from the peripheral blood have been shown to be able to differentiate
into numerous cell lineages. However, adults have only a small number of these
circulating stem cells. The aim of the present study was to assess a new
isolation and enrichment technique for CD133+ stem cells from peripheral blood
with the use of Percoll density gradients. Our results demonstrated the presence
of two large mononuclear bands when whole blood was centrifuged with 48% and
50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major
CD133+ enrichment at the 48% Percoll concentration in one of the two bands.
Further culture of these cells resulted in the formation of multiple
colony-forming units. Our results suggest an advantage from using a simple
Percoll gradient for successful CD133+ cell recovery, which could aid in
differentiation and transplantation protocols.</span> 
</p>
%K Stem Cells Recovery
%K CD133+
%K Percoll
%K Density Gradients
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47556