%0 Journal Article
%T Clinical Application of T-SPOT.TB Using Pleural Effusion as a Diagnostic Method for Tuberculosis Infection
%A Yoshihiro Kobashi
%A Keiji Mouri
%A Yasushi Obase
%A Shigeki Kato
%A Mikio Oka
%J Open Journal of Respiratory Diseases
%P 64-72
%@ 2163-9418
%D 2014
%I Scientific Research Publishing
%R 10.4236/ojrd.2014.42010
%X <div align=\"justify\">
	Introduction: The objective of this study was the comparison of the results of T-SPOT.TB using pleural effusion (PE) with those of IGRAs using peripheral blood (PB) or other diagnostic methods for the diagnosis of tuberculous (TB) pleurisy. Methods: We measured adenosine deaminase (ADA) in PE, QuantiFERON TB-Gold In-Tube (QFT), and T-SPOT.TB using PB, and T-SPOT.TB using PE. The definite group of TB pleurisy included 12 patients and other disease group 33 patients. Main find-ings: Sensitivity for QFT using PB was 83% and specificity was 85%, sensitivity for T-SPOT.TB using PB was 92% and specificity was 82%, while sensitivity for ADA in PE was 83% and specificity was 76%. When we adopted the same cut-off level of a positive response for T-SPOT.TB as PB using PE, sensitivity for T-SPOT.TB using PE was 100% and specificity was 82%, respectively. Although there were no significant differences among the four diagnostic methods, sensitivity for T-SPOT.TB using PE gave the most accurate diagnosis of TB-definite patients compared to ADA in PE or QFT using PB. Conclusions: If we performed T-SPOT.TB using a local specimen from the infection site, we could obtain a higher sensitivity than IGRAs using PB or ADA in PE and the numbers of ESAT-6 and CFP-10-positive SFCs were 3 to 5 fold higher in PEMCs than in PBMCs. T-SPOT.TB using PE may become a useful diagnostic method for TB pleurisy.
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%K T-SPOT.TB
%K QuantiFERON
%K Adenosine Deaminase
%K Pleural Effusion (PE)
%K Peripheral Blood (PB)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=45581