%0 Journal Article
%T IL-1 Receptor Type II Production Is Upregulated by IL-4 and IL-13, and Downregulated by IFN-<i>¦Ã</i> in Mouse Gingival Epithelial Cells
%A Y. Kamiya
%A Y. Ishihara
%A H. Kamei
%A Y. Ozawa
%A H. Mizutani
%A K. Kubo
%A H. Maeda
%A T. Noguchi
%J Modern Research in Inflammation
%P 37-47
%@ 2169-9690
%D 2014
%I Scientific Research Publishing
%R 10.4236/mri.2014.32005
%X <div style=\"text-align:justify;\">
	<p class=\"MsoNormal\">
		<span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">Background and Objective: Interleukin-1 (IL-1) binds to 2 distinct
and separate receptors, types I and II (IL-1RI and IL-1RII, respectively). The
binding of IL-1 to IL-1RI induces cellular signaling and biological effects,
whereas binding to IL-1RII does not induce cellular signaling and indirectly
inhibits IL-1 biological activities such as that of the decoy receptor.
Recently, Suzukiet al.reported that soluble IL-1RII (sIL-1RII) was detected in
gingival crevicular fluid from a periodontitis patient. However, it remains
unclear which cells produce sIL-1RII detected in periodontal tissues. We
examined the localization of IL-1RII producing cells in gingival tissues as
well as related production control mechanisms. Material and Methods: IL-1RII
mRNA expression in gingival epithelial cells (GE1) was performed by real-time
PCR analysis, while the amount of sIL-1RII production in supernatant from GE1
cells was examined by dot-blot analysis. Involvement of the phosphorylation of
STAT6 in the signaling pathway was determined by western blot analysis.
Statistical analysis was performed with Student¡¯st-test. Results: Culturing
with IL-4 and IL-13 significantly increased IL-1RII mRNA to levels 10.5-and
8.89-fold, respectively, above that of the control (p&lt; 0.01), while addition
of interferon-</span><em><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">¦Ã</span></em><span class=\"apple-converted-space\" style=\"font-family:Verdana;\"><i><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\"> </span></i></span><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">(IFN-</span><em><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">¦Ã</span></em><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">) significantly suppressed IL-1RII mRNA by 0.22-fold as
compared to the control (p&lt; 0.05). Soluble IL-1RII in the supernatant of
cultured GE1 cells was increased by IL-4 and IL-13, and decreased by IFN-</span><em><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">¦Ã</span></em><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">, while western blotting determines the suppression of
IL-1RII production by IFN-</span><em><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">¦Ã</span></em><span style=\"font-size:5.5pt;font-family:Verdana;\" lang=\"EN-US\">. Without the addition of IL-4 or IL-13 with or without
%K IL-1RII
%K IL-4
%K IL-13
%K IFN-&lt
%K i&gt
%K ¦Ã&lt
%K /i&gt
%K STAT-6
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=45078