%0 Journal Article
%T Construction of Vector for Functional  Analysis of the Intrinsically Bent DNA  in the ACE3 Replicator from  <i>Drosophila melanogaster</i>
%A Douglas Vin¨ªcius Bassalobre de Freitas
%A Fabr¨ªcia Gimenes
%A Adriana Fiorini
%A Fabiana dos Santos Rando
%A Quirino Alves de Lima Neto
%A Maria Aparecida Fernandez
%J Open Journal of Genetics
%P 8-15
%@ 2162-4461
%D 2014
%I Scientific Research Publishing
%R 10.4236/ojgen.2014.41002
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:10.0pt;line-height:100%;font-family:;\" \"=\"\"><span style=\"font-family:Verdana;line-height:1.5;\">DNA replication is a crucial process for species
survival, nevertheless it is not clear which factors define origin selection in
multicellular eukaryotes. Developmental gene amplification systems, such as the
one described during ovarian follicles development in </span><i><span style=\"font-family:Verdana;line-height:1.5;\">Drosophila melanogaster</span></i><span style=\"font-family:Verdana;line-height:1.5;\">,</span><i> </i><span style=\"font-family:Verdana;line-height:1.5;\">are
useful tools for studying of DNA replication process in these organisms. We
previously described that the well characterized third chromosome amplified
domain of </span><i><span style=\"font-family:Verdana;line-height:1.5;\">D. melanogater </span></i><span style=\"font-family:Verdana;line-height:1.5;\">displays
three intrinsically bent DNA sites: </span><i><span style=\"font-family:Verdana;line-height:1.5;\">b</span></i><span style=\"font-family:Verdana;line-height:1.5;\">1,
localized at an amplification control element (ACE3), </span><i><span style=\"font-family:Verdana;line-height:1.5;\">b</span></i><span style=\"font-family:Verdana;line-height:1.5;\">2 and </span><i><span style=\"font-family:Verdana;line-height:1.5;\">b</span></i><span style=\"font-family:Verdana;line-height:1.5;\">3, both localized
at the preferential origin ori-</span><i><span style=\"font-family:Verdana;line-height:1.5;\">¦Â</span></i><span style=\"font-family:Verdana;line-height:1.5;\">.
This proposal aimed to construct a </span><i><span style=\"font-family:Verdana;line-height:1.5;\">Drosophila</span></i><span style=\"font-family:Verdana;line-height:1.5;\"> transformation vector, which contains a short deletion at the ACE3, in order to
reduce the intrin</span></span><span style=\"font-size:10.0pt;line-height:100%;font-family:;\" \"=\"\"><span style=\"font-family:Verdana;line-height:1.5;\">sically bent DNA site </span><i><span style=\"font-family:Verdana;line-height:1.5;\">b</span></i><span style=\"font-family:Verdana;line-height:1.5;\">1, and analyze the functional role of
this site in the gene amplification process. Through a series of cloning steps,
we obtained a Big Parent vector derivative, containing a deletion at the
positions 176</span></span><span style=\"font-size:10pt;line-height:1.5;font-family:Verdana;\">-</span><span style=\"font-size:10.0pt;line-height:100%;font-family:;\" \"=\"\"><span
%K DNA Replication
%K Gene Amplification
%K ACE3
%K Intrinsically Bent DNA
%K &lt
%K i&gt
%K Drosophila melanogaster&lt
%K /i&gt
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=43456