%0 Journal Article
%T Reduction of pro-inflammatory cytokines in rats following 7-day oral supplementation with a proprietary eggshell membrane-derived product
%A Kevin J. Ruff
%A Dale P. DeVore
%J Modern Research in Inflammation
%P 19-25
%@ 2169-9690
%D 2014
%I Scientific Research Publishing
%R 10.4236/mri.2014.31003
%X NEM<sup>®</sup> brand
eggshell membrane is a novel dietary supplement that has been clinically shown
to alleviate arthritis joint pain and stiffness; however the mechanism of
action is not well understood. Preliminary evidence from an in vitro study of NEM<SUP>®</SUP> indicated that the mechanism of action
may be based on the reduction of pro-inflammatory cytokines. In vivo studies were
therefore initiated to evaluate the effects of NEM<SUP>®</SUP> on pro-inflammatory and
anti-inflammatory cytokines following oral administration in rats. NEM<SUP>®</SUP> was administered daily at doses of
6.13 mg/kg bw/day (Study 1), 10.0 mg/kg bw/day (Study 2), or at doses of 0
(control), 26.0 or 52.0 mg/kg bw/day (Study 3) by oral gavage for 7 consecutive
days. Inflammation was induced in the Study 3 rats by intraperitoneal injection
of lipopolysaccharide. Changes in plasma cytokine levels from baseline
following 7 days of oral supplementation with NEM<SUP>®</SUP> at 6.13 mg/kg bw/ day (Study 1) were
statistically significant at Day 8 for IL-2, TIMP-1 and VEGF, at Day 21 for
IL-10, and at Day 35 for MCP-1, MCP-3 and TIMP-1, and at 10.0 mg/kg
%K Pro-Inflammatory Cytokines
%K Eggshell Membrane
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=43071