%0 Journal Article
%T Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions
%A Mambo Moyo
%J Open Journal of Applied Biosensor
%P 1-7
%@ 2168-5398
%D 2014
%I Scientific Research Publishing
%R 10.4236/ojab.2014.31001
%X Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn<sup>2+</sup> in aqueous solution. The biosensor designed was able to determine Zn<sup>2+</sup> in the range of 0.35 - 12 mg/L with a detection limit of 7.5 ¦Ìg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.
%K Horseradish Peroxidase
%K Maize Tassel
%K Multiwalled Carbon Nanotubes
%K Metal Biosensor
%K Zn&lt
%K sup&gt
%K 2+&lt
%K /sup&gt
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=42944