%0 Journal Article
%T A Green Approach towards the Synthesis of Enantio Pure Diols Using Horse Radish Peroxidase Enzyme Immobilized on Magnetic Nanoparticles
%A Seelam Siva Deepthi
%A Ernala Prasad
%A Basireddy Venkata Subba Reddy
%A Bojja Sreedhar
%A Adari Bhaskar Rao
%J Green and Sustainable Chemistry
%P 15-19
%@ 2160-696X
%D 2014
%I Scientific Research Publishing
%R 10.4236/gsc.2014.41003
%X <p class=\"MsoNormal\">
	<span lang=\"EN-US\">Enantiopure
epoxides and their corresponding chiral vicinal diols serve as valuable
intermediates in the synthe</span><span lang=\"EN-US\">sis of
biologically active pharma and agro-compounds and also value added fine
chemicals. Biocatalysts are well known for their selective hydrolysis of
racemic epoxides to give optically pure chiral diols. This study highlights an
efficient process of synthesis of chiral vicinal diols in good yields and </span><span lang=\"EN-US\">enantioselectiviy using horse radish pe</span><span lang=\"EN-US\">roxidase enzyme immobilized on the amine functionalized magnetic
nano particles (Fe<sub>3</sub>O<sub>4</sub> nanoparticles) as enzyme carriers</span><span lang=\"EN-US\">. It</span><span lang=\"EN-US\"> also facilitates separation of
MNP-immobilized enzymes by applying external magnetic field. The immobilization
of magnetic nano particles was confirmed by transmission electron microscope
(TEM) and scanning electron microscope (SEM). The MNP-immobilized peroxidase
enzyme improved stability of the en</span><span lang=\"EN-US\">zyme and has shown broader substrate specificity in enantioselective
hydrolysis of racemic epoxides, under mild and environmentally friendly
conditions. The methodology MNP-immobilized enzyme developed in the synthesis
of chiral diols has a potential </span><span lang=\"EN-IN\">for use in large</span><span lang=\"EN-IN\">-</span><span lang=\"EN-IN\">scale applications</span><span lang=\"EN-US\">.</span> 
</p>
%K Magnetic Nanoparticles
%K Horseradish Peroxidase
%K Immobilization
%K Vicinal-Diols
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=42859