%0 Journal Article
%T Ultrastructural Analysis of in Vitro Adherence and Production of Acid Proteases by Clinical Isolates of <i>Candida parapsilosis</i> Sensu Stricto Following Growth in the Presence of Keratinous Substrates from Human Source
%A Ana Fl¨˘via L. Specian
%A Luciana Furlaneto-Maia
%A C¨Ślia G. T. J. Andrade
%A Marcia Cristina Furlaneto
%J Advances in Microbiology
%P 1-6
%@ 2165-3410
%D 2013
%I Scientific Research Publishing
%R 10.4236/aim.2013.38A001
%X <p class=\"MsoNormal\" style=\"text-align:justify;\">
	<i><span style=\"font-family:Verdana;\" lang=\"EN-US\">Candida parapsilosis</span></i><span style=\"font-family:Verdana;\" lang=\"EN-US\"> is an increasingly
important human pathogen. However, </span><span style=\"font-family:Verdana;\" lang=\"EN-GB\">little is known about its potential to cause disease. </span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">The aims of the present study were
to </span><span style=\"font-family:Verdana;\" lang=\"EN-GB\">analyse the
production of acid proteinases by clinical isolates of </span><i><span style=\"font-family:Verdana;\" lang=\"EN-CA\">C. parapsilosis</span></i><span style=\"font-family:Verdana;\" lang=\"EN-GB\"> in the presence of different
keratinous substrates from human sources (</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">stratum corneum, </span><span style=\"font-family:Verdana;\" lang=\"EN-GB\">nail and hair) </span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">and to </span><span style=\"font-family:Verdana;\" lang=\"EN-GB\">verify the capability of yeast cells to adhere
and grow as biofilm on these substrates</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">. By scanning electron microscopy</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">,</span><span lang=\"EN-CA\"><span style=\"font-family:Verdana;\"> it was observed that all </span><i><span style=\"font-family:Verdana;\">C.
parapsilosis</span></i><span style=\"font-family:Verdana;\"> sensu stricto isolates adhered to the keratinous substrates</span></span><span style=\"font-family:Verdana;\" lang=\"EN-GB\">. For t</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">he isolate </span><span style=\"font-family:Verdana;\" lang=\"EN-GB\">recovered</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\"> from onychomycosis</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">,</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\"> the cell population attached to
stratum corneum and hair keratin consisted mainly of blastoconidia.
Differently, on nail keratin</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\">,</span><span style=\"font-family:Verdana;\" lang=\"EN-CA\"> pseudohyphae production was observed. Overall, there was a loose association
between yeast cells and</span><span style=\"font-family:Verdana;\" lang=\"EN-GB\"> keratinous substrates. However, on stratum corneum</span><span style=\"font-family:Verdana;\" lang=\"EN-GB\">,</span><span style=\"font-family:Verdana;\" lang=\"EN-GB\"> flocculent extracellular material
was seen
%K Adherence
%K Keratinic Substrates
%K SEM
%K Acid Proteinase
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=40728