%0 Journal Article
%T Rapid Detection Method for Enteroaggregative <i>Escherichia coli</i> Using Simple Clump Formation and Aggregative Assay
%A Miyuki Fujioka
%A Chowdhury Rafiqul Ahsan
%A Yoshimitsu Otomo
%J Advances in Microbiology
%P 552-556
%@ 2165-3410
%D 2013
%I Scientific Research Publishing
%R 10.4236/aim.2013.38074
%X <p style=\"text-align:justify;\">
	<span><span style=\"font-family:Verdana;\">Enteroaggregative </span><i><span style=\"font-family:Verdana;\">Escherichia
coli</span></i><span style=\"font-family:Verdana;\"> (EAggEC) strains cause the persistent diarrhea in infants and
compromised hosts in developing countries. These strains are currently defined
as </span><i><span style=\"font-family:Verdana;\">E. coli</span></i><span style=\"font-family:Verdana;\"> that adheres to HEp-2
cells in an aggregative adherence (AA) pattern. In this study, we compared 4
different rapid methods for the detection of EAggEC using a PCR assay, clump
formation test, glass slide adherence assay, and the HEp-2 cell adherence
assay. Out of 683 </span><i><span style=\"font-family:Verdana;\">E. coli</span></i><span style=\"font-family:Verdana;\"> strains
isolated from diarrheal stool samples, we detected 17 </span><i><span style=\"font-family:Verdana;\">aggR</span></i><span style=\"font-family:Verdana;\"> and/or clump-positive strains, and identified 2 </span><i><span style=\"font-family:Verdana;\">aggR</span></i><span style=\"font-family:Verdana;\">-positive, clump-negative strains
and 2 </span><i><span style=\"font-family:Verdana;\">aggR</span></i><span style=\"font-family:Verdana;\">-negative, clump-positive
strains. All the </span><i><span style=\"font-family:Verdana;\">aggR </span></i><span style=\"font-family:Verdana;\">positive and
clump positive strains also showed positive results in glass slide adherence
and HEp-2 cell adherence assays. From all these results, we suggest the
following procedure for the rapid identification of EAggEC strains: first,
screen </span><i><span style=\"font-family:Verdana;\">E. coli</span></i><span style=\"font-family:Verdana;\"> strains with the clump
formation test and subsequently perform the glass slide adherence assay to
observe AA for confirmation.</span></span><span></span> 
</p>
<p style=\"text-align:justify;\">
	<span style=\"font-family:Verdana;\"> </span> 
</p>
%K &lt
%K i&gt
%K Escherichia coli&lt
%K /i&gt
%K Aggregative Adherence
%K Clump Formation
%K Rapid Detection
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=40801