%0 Journal Article
%T New Stability Indicating Method for Quantification of  Impurities in Amlodipine and Benazepril Capsules by  Validated HPLC
%A Rama Joga Venkata Eranki
%A Gopichand Inti
%A Venkatasubramanian Jayaraman
%A Raghuram Pannala
%A Sudhakar Rao Vidiyala
%A Jadi Sree Ramulu
%J American Journal of Analytical Chemistry
%P 715-724
%@ 2156-8278
%D 2013
%I Scientific Research Publishing
%R 10.4236/ajac.2013.412086
%X <p class=\"MsoNormal\" style=\"text-align:justify;\">
	<span lang=\"EN-US\"><span style=\"font-family:Verdana;\">A stability indicating LC method was developed for the
simultaneous determination of Amlodipine and Benazepril capsules in
pharmaceutical dosage form. Efficient chromatographic separation was achieved
on Symmetry C</span><sub><span style=\"font-family:Verdana;\">18</span></sub><span style=\"font-family:Verdana;\"> stationary phase with simple combination of amobile
phase containing 750 mL of DI Water, 250 mL of Acetonitrile and 2 mL of
Octylamine into suitable container with adjusted pH to 2.50 ¡À</span></span><span lang=\"EN-US\"> </span><span lang=\"EN-US\"><span style=\"font-family:Verdana;\">0.05
with the aid of Ortho phosphoric acid delivered in an isocratic mode and
quantification was carried out using UV detection at 240 nm at a flow rate of
1.0 mL<span style=\"white-space:nowrap;\">¡¤</span>min<sup>-</sup></span><sup><span style=\"font-family:Verdana;\">1</span></sup><span style=\"font-family:Verdana;\"> with </span></span><span lang=\"EN-US\" style=\"font-family:Verdana;\">an </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">injection volume of 20 ¦Ìl and ambient column
temperature.</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">This</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">method is capable to detect both the drug components of Amlodipine
and Benazepril in presence of their degradation products (Amlodipine Imp-A and
Benazepril Impurity-C) with</span><span lang=\"EN-US\" style=\"font-family:Verdana;\"> a</span><span lang=\"EN-US\" style=\"font-family:Verdana;\"> detection level of 0.05%.</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">Amlodipine/Benazepril
in their combination drug product were exposed to thermal, photolytic,
hydrolytic and oxidative stress conditions, and the samples were analysed. Peak
homogeneity data of Amlodipine and Benazeprilis </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">were </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">obtained using
PDA detector, in the stressed sample chromatograms, demonstrating the
specificity. The method shows excellent linearity over a range of 0.05</span><span lang=\"EN-US\" style=\"font-family:Verdana;\">%-</span><span lang=\"EN-US\" style=\"font-family:Verdana;\">2.0%
for Amlodipine, Amlodipine Impurity-A and 0.05</span><span lang=\"EN-US\" style=\"font-family:Verdana;\">%-</span><span lang=\"EN-US\"
%K Column Liquid Chromatography
%K Method Validation
%K Stability Indicating Study Amlodipine and Benazepril
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=40394