%0 Journal Article
%T Gene Expression Analysis of the IPEC-J2 Cell Line: A Simple Model for the Inflammation-Sensitive Preterm Intestine
%A Ann Cathrine F. St£¿y
%A Peter M. H. Heegaard
%A Per T. Sangild
%A Mette V. £¿stergaard
%A Kerstin Skovgaard
%J ISRN Genomics
%D 2013
%R 10.1155/2013/980651
%X The IPEC-J2 cell line was studied as a simple model for investigating responses of the newborn intestinal epithelium to diets. Especially, the small intestine of immature newborns is sensitive to diet-induced inflammation. We investigated gene expression of epithelial- and immune response-related genes in IPEC-J2 cells stimulated for 2£¿h with milk formula (CELL-FORM), colostrum (CELL-COLOS), or growth medium (CELL-CONTR) and in distal small intestinal tissue samples from preterm pigs fed milk formula (PIG-FORM) or colostrum (PIG-COLOS). High throughput quantitative PCR analysis of 48 genes revealed the expression of 22 genes in IPEC-J2 cells and 31 genes in intestinal samples. Principal component analysis (PCA) discriminated the gene expression profile of IPEC-J2 cells from that of intestinal samples. The expression profile of intestinal tissue was separated by PCA into 2 groups according to diet, whereas no diet-dependent grouping was seen for IPEC-J2 cells. Expression differences between PIG-FORM and PIG-COLOS were found for DEFB1, CXCL10, IL1RN, and ALPI, while IL8 was upregulated in CELL-FORM compared with CELL-CONTR. These differences, between IPEC-J2 cells and intestinal tissue from preterm pigs, both used as models for the newborn intestine, underline that caution must be exercised prior to analysis and interpretation of diet-induced effects on gene expression. 1. Introduction The intestine is the site for nutrient digestion and absorption, but also a major immunological defense barrier that recognizes and responds to external antigens. In addition to the gut mucosal immune system, intestinal epithelial cells (IECs) are involved in the initiation and coordination of the intestinal immune response by the production of signaling molecules including cytokines and chemokines [1]. The IPEC-J2 cell line originates from the jejunum of an unsuckled neonatal pig [2] and is morphologically and functionally similar to IECs with microvilli and tight junctions as well as expression and production of cytokines, defensins, toll-like receptors, and mucins [2¨C4]. It has previously been used to investigate host-pathogen interactions and immune responses with relevance for human and swine intestinal diseases [2, 3, 5¨C8] and could be a simple in vitro model to investigate the immune response of IECs in newborns. Necrotizing enterocolitis (NEC) is a serious gastrointestinal disease in preterm infants arising from the combined effect of prematurity, abnormal bacterial colonization, and enteral feeding. In particularly, feeding with milk formula predisposes to NEC,
%U http://www.hindawi.com/journals/isrn.genomics/2013/980651/