%0 Journal Article
%T Prevalence of Hepatitis C Virus Genotypes in District Bannu, Khyber Pakhtunkhwa, Pakistan
%A Shamim Saleha
%A Anwar Kamal
%A Farman Ullah
%A Nasar Khan
%A Asif Mahmood
%A Sanaullah Khan
%J Hepatitis Research and Treatment
%D 2014
%I Hindawi Publishing Corporation
%R 10.1155/2014/165826
%X Determination of an individual¡¯s hepatitis C virus (HCV) genotypes prior to antiviral therapy has become increasingly important for the clinical management and prognosis of HCV infection. Therefore, this study was conducted to investigate the prevalence of HCV genotypes in HCV infected patients of district Bannu in Khyber Pakhtunkhwa region of Pakistan. Serum samples of 117 seropositive patients were screened for HCV-RNA by using reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) and then PCR positive samples were subjected to HCV genotyping. Out of 117 seropositive samples, 110 samples were found positive by PCR analysis. Genotype 3a was the most prevalent one detected in 38% of patients, followed by genotype 3b in 21% of patients, and then genotype 2a in 12% of patients. However 21% of HCV-PCR positive samples could not be genotyped by method used in this study. Genotype 3a was the most prevalent genotype in patients of all age groups and its prevalence was found high among patients with increasing age (>34 years). Moreover, genotypes 3a and 3b were found to be the most prevalent genotypes in patients with history of shaving by barbers, receiving multiple injections, and dental procedures. In conclusion there is need of further investigation of genotypes of HCV by using more sensitive assays and considering large sample size in district Bannu. 1. Introduction HCV infection is among life threatening public health problems worldwide, with over 170¨C200 million infected people [1] including about 17 million from Pakistan [2]. HCV is considered the leading cause of liver cirrhosis and hepatocellular carcinoma. It has been estimated to cause approximately 27% of cirrhosis and 25% of hepatocellular carcinoma cases worldwide [3]. Each year about 350,000 people die due to HCV [4]. HCV is a small enveloped, positive sense single stranded RNA virus and has been classified as a separate genus hepacivirus in the Flaviviridae family [5]. The HCV genome is approximately 9.6£¿kb, encoding a polyprotein of about 3010 amino acids and is flanked by short untranslated regions (UTRs) regions at the 5¡ä and 3¡ä terminus [6]. This polyprotein is posttranslationally processed by viral and cellular proteins to generate the structural proteins (C, E1, E2, and p7) and nonstructural proteins (NS2, NS3, NS4A, NS4B, NS5A, and NS5B), [7]. HCV shows high degree of genetic heterogeneity; consequently six major genotypes and multiple subtypes of HCV have been identified so far in world [8]. Distribution of HCV genotypes and subtypes in different regions of the world
%U http://www.hindawi.com/journals/heprt/2014/165826/