%0 Journal Article
%T Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study
%A Jun Yang
%A Christopher D. d'Esterre
%A Zareen Amtul
%A David F. Cechetto
%A Ting Yim Lee
%J PLOS ONE
%D 2014
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0100575
%X Background/Objective Clinical evidence indicates that cerebral ischemia (CI) and a pathological factor of Alzheimer's disease, the ¦Â-amyloid (A¦Â) protein, can increase the rate of cognitive impairment in the ageing population. Using the CT Perfusion (CTP) functional imaging, we sought to investigate the interaction between CI and the A¦Â protein on cerebral hemodynamics. Methods A previously established rat model of CI and A¦Â was used for the CTP study. Iodinated contrast was given intravenously, while serial CT images of sixteen axial slices were acquired. Cerebral blood flow (CBF) and blood volume (CBV) parametric maps were co-registered to a rat brain atlas and regions of interest were drawn on the maps. Microvascular alteration was investigated with histopathology. Results CTP results revealed that ipsilateral striatum of A¦Â+CI and CI groups showed significantly lower CBF and CBV than control at the acute phase. Striatal CBF and CBV increased significantly at week 1 in the CI and A¦Â+CI groups, but not in the A¦Â alone or control group. Histopathology showed that average density of dilated microvessels in the ipsilateral striatum in CI and A¦Â+CI groups was significantly higher than control at week 1, indicating this could be associated with hyperperfusion and hypervolemia observed from CTP results. Conclusion These results demonstrate that CTP can quantitatively measure the hemodynamic disturbance on CBF and CBV functional maps in a rat model of CI interacting with A¦Â.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0100575