%0 Journal Article
%T Fructose-Asparagine Is a Primary Nutrient during Growth of Salmonella in the Inflamed Intestine
%A Mohamed M. Ali
%A David L. Newsom
%A Juan F. Gonz¨¢lez
%A Anice Sabag-Daigle
%A Christopher Stahl
%A Brandi Steidley
%A Judith Dubena
%A Jessica L. Dyszel
%A Jenee N. Smith
%A Yakhya Dieye
%A Razvan Arsenescu
%A Prosper N. Boyaka
%A Steven Krakowka
%A Tony Romeo
%A Edward J. Behrman
%A Peter White
%A Brian M. M. Ahmer
%J PLOS Pathogens
%D 2014
%I Public Library of Science (PLoS)
%R doi/10.1371/journal.ppat.1004209
%X Salmonella enterica serovar Typhimurium (Salmonella) is one of the most significant food-borne pathogens affecting both humans and agriculture. We have determined that Salmonella encodes an uptake and utilization pathway specific for a novel nutrient, fructose-asparagine (F-Asn), which is essential for Salmonella fitness in the inflamed intestine (modeled using germ-free, streptomycin-treated, ex-germ-free with human microbiota, and IL10£¿/£¿ mice). The locus encoding F-Asn utilization, fra, provides an advantage only if Salmonella can initiate inflammation and use tetrathionate as a terminal electron acceptor for anaerobic respiration (the fra phenotype is lost in Salmonella SPI1£¿ SPI2£¿ or ttrA mutants, respectively). The severe fitness defect of a Salmonella fra mutant suggests that F-Asn is the primary nutrient utilized by Salmonella in the inflamed intestine and that this system provides a valuable target for novel therapies.
%U http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1004209