%0 Journal Article
%T Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides)
%A Shuhong Ouyang
%A Dong Zhang
%A Jun Han
%A Xiaojie Zhao
%A Yu Cui
%A Wei Song
%A Naxin Huo
%A Yong Liang
%A Jingzhong Xie
%A Zhenzhong Wang
%A Qiuhong Wu
%A Yong-Xing Chen
%A Ping Lu
%A De-Yun Zhang
%A Lili Wang
%A Hua Sun
%A Tsomin Yang
%A Gabriel Keeble-Gagnere
%A Rudi Appels
%A Jaroslav Dole£żel
%A Hong-Qing Ling
%A Mingcheng Luo
%A Yongqiang Gu
%A Qixin Sun
%A Zhiyong Liu
%J PLOS ONE
%D 2014
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0100160
%X Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval. Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6, Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a framework for the physical mapping and map-based cloning of MlIW172.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0100160