%0 Journal Article
%T Temperature Responses of Ammonia-Oxidizing Prokaryotes in Freshwater Sediment Microcosms
%A Jin Zeng
%A Dayong Zhao
%A Zhongbo Yu
%A Rui Huang
%A Qinglong L. Wu
%J PLOS ONE
%D 2014
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0100653
%X In order to investigate the effects of temperature on the abundances and community compositions of ammonia-oxidizing archaea (AOA) and bacteria (AOB), lake microcosms were constructed and incubated at 15ˇăC, 25ˇăC and 35ˇăC for 40 days, respectively. Temperature exhibited different effects on the abundance and diversity of archaeal and bacterial amoA gene. The elevated temperature increased the abundance of archaeal amoA gene, whereas the abundance of bacterial amoA gene decreased. The highest diversity of bacterial amoA gene was found in the 25ˇăC treatment sample. However, the 25ˇăC treatment sample maintained the lowest diversity of archaeal amoA gene. Most of the archaeal amoA sequences obtained in this study affiliated with the Nitrosopumilus cluster. Two sequences obtained from the 15ˇăC treatment samples were affiliated with the Nitrosotalea cluster. N. oligotropha lineage was the most dominant bacterial amoA gene group. Several sequences affiliated to Nitrosospira and undefined N. europaea/NC. mobilis like lineage were found in the pre-incubation and 25ˇăC treatment groups.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0100653