%0 Journal Article
%T Coumarin-Based Fluorescent Probes for Dual Recognition of Copper(II) and Iron(III) Ions and Their Application in Bio-Imaging
%A Olimpo Garc¨ªa-Beltr¨¢n
%A Bruce K. Cassels
%A Claudio P¨¦rez
%A Natalia Mena
%A Marco T. N¨²£¿ez
%A Natalia P. Mart¨ªnez
%A Paulina Pavez
%A Margarita E. Aliaga
%J Sensors
%D 2014
%I MDPI AG
%R 10.3390/s140101358
%X Two new coumarin-based ¡°turn-off¡± fluorescent probes, ( E)-3-((3,4-dihydroxybenzylidene)amino)-7-hydroxy-2 H-chromen-2-one ( BS1) and ( E)-3-((2,4-dihydroxybenzylidene)amino)-7-hydroxy-2 H-chromen-2-one ( BS2), were synthesized and their detection of copper(II) and iron(III) ions was studied. Results show that both compounds are highly selective for Cu 2+ and Fe 3+ ions over other metal ions. However, BS2 is detected directly, while detection of BS1 involves a hydrolysis reaction to regenerate 3-amino-7-hydroxycoumarin ( 3) and 3,4-dihydroxybenzaldehyde, of which 3 is able to react with copper(II) or iron(III) ions. The interaction between the tested compounds and copper or iron ions is associated with a large fluorescence decrease, showing detection limits of ca. 10 £¿5 M. Preliminary studies employing epifluorescence microscopy demonstrate that Cu 2+ and Fe 3+ ions can be imaged in human neuroblastoma SH-SY5Y cells treated with the tested probes.
%K Cu2+ ion
%K Fe3+ ion
%K turn-off probes
%K coumarin-based probes
%K fluorescence sensors
%K bio-imaging
%U http://www.mdpi.com/1424-8220/14/1/1358