%0 Journal Article
%T Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis
%A Maria-Cristina Radulescu
%A Bogdan Bucur
%A Madalina-Petruta Bucur
%A Gabriel Lucian Radu
%J Sensors
%D 2014
%I MDPI AG
%R 10.3390/s140101028
%X A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h £¿1 with an RSD of 2.7%. The detection limits for both batch and FIA measurements were 0.1 ¦ÌM for methanol and 0.2 ¦ÌM for aspartame, respectively. The enzymatic biosensor was successfully applied for aspartame determination in different sample matrices/commercial products (liquid and solid samples) without any pre-treatment step prior to measurement.
%K alcohol oxidase
%K carboxyl esterase
%K flow injection analysis
%K aspartame
%U http://www.mdpi.com/1424-8220/14/1/1028