%0 Journal Article
%T Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer
%A Noorsharmimi Omar
%A Qiuting Loh
%A Gee Jun Tye
%A Yee Siew Choong
%A Rahmah Noordin
%A J£¿rn Gl£¿kler
%A Theam Soon Lim
%J Sensors
%D 2014
%I MDPI AG
%R 10.3390/s140100346
%X G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.
%K G-quadruplex
%K DNAzyme
%K gold nanoparticles
%K antibody
%U http://www.mdpi.com/1424-8220/14/1/346