%0 Journal Article
%T Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9
%A Jian Woon Chen
%A Chong-Lek Koh
%A Choon-Kook Sam
%A Wai-Fong Yin
%A Kok-Gan Chan
%J Sensors
%D 2013
%I MDPI AG
%R 10.3390/s131013217
%X In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N¨Chexanoylhomoserine lactone (C6-HSL) and N¨Coctanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
%K CepI/CepR homologs
%K MALDI-TOF mass spectrometry
%K N-hexanoylhomoserine lactone
%K tropical soil bacteria
%K triple quadrupole LC/MS
%U http://www.mdpi.com/1424-8220/13/10/13217