%0 Journal Article
%T A Low Density Microarray Method for the Identification of Human Papillomavirus Type 18 Variants
%A Thuluz Meza-Menchaca
%A John Williams
%A Roc¨ªo B. Rodr¨ªguez-Estrada
%A Aracely Garc¨ªa-Bravo
%A ¨¢ngel Ramos-Ligonio
%A Aracely L¨®pez-Monteon
%A Rossana C. Zepeda
%J Sensors
%D 2013
%I MDPI AG
%R 10.3390/s131012975
%X We describe a novel microarray based-method for the screening of oncogenic human papillomavirus 18 (HPV-18) molecular variants. Due to the fact that sequencing methodology may underestimate samples containing more than one variant we designed a specific and sensitive stacking DNA hybridization assay. This technology can be used to discriminate between three possible phylogenetic branches of HPV-18. Probes were attached covalently on glass slides and hybridized with single-stranded DNA targets. Prior to hybridization with the probes, the target strands were pre-annealed with the three auxiliary contiguous oligonucleotides flanking the target sequences. Screening HPV-18 positive cell lines and cervical samples were used to evaluate the performance of this HPV DNA microarray. Our results demonstrate that the HPV-18¡¯s variants hybridized specifically to probes, with no detection of unspecific signals. Specific probes successfully reveal detectable point mutations in these variants. The present DNA oligoarray system can be used as a reliable, sensitive and specific method for HPV-18 variant screening. Furthermore, this simple assay allows the use of inexpensive equipment, making it accessible in resource-poor settings.
%K HPV-18
%K microarray
%K variants
%K LCR
%U http://www.mdpi.com/1424-8220/13/10/12975