%0 Journal Article
%T Validation of a Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry Method for Determination of All-Trans Retinoic Acid in Human Plasma and Its Application to a Bioequivalence Study
%A Jing-Bo Peng
%A Chen-Hui Luo
%A Yi-Cheng Wang
%A Wei-Hua Huang
%A Yao Chen
%A Hong-Hao Zhou
%A Zhi-Rong Tan
%J Molecules
%D 2014
%I MDPI AG
%R 10.3390/molecules19011189
%X A sensitive, reliable and specific LC-MS-MS method was developed and validated for the identification and quantitation of all- trans retinoic acid (ATRA) in human plasma. Acitretin was used as the internal standard (IS). After liquid-liquid extraction of 500 米L plasma with methyl tert-butyl ether (MTBE), ATRA and the IS were chromatographed on a HyPURITY C18 column (150 mm ℅ 2.1 mm, 5 米m) with the column temperature set at 40 ∼C. The mobile phase was consisted of 40% phase A (MTBE每methanol每acetic acid, 50:50:0.5, v/v) and 60% phase B (water每methanol每acetic acid, 50:50:0.5, v/v) with a flow rate of 0.3 mL/min. The API 4000 triple quadrupole mass spectrometer was operated in multiple reaction monitoring (MRM) mode via the positive electrospray ionization interface using the transition m/z 301.4 ↙ 123.1 for ATRA and m/z 326.9 ↙ 177.1 for IS, respectively. The calibration curve was linear over the range of 0.45每217.00 ng/mL (r ≡ 0.999) with a lower limit of quantitation (LLOQ) of 0.45 ng/mL. The intra- and inter-day precisions values were below 8% relative standard deviation and the accuracy was from 98.98% to 106.19% in terms of relative error. The validated method was successfully applied in a bioequivalence study of ATRA in Chinese healthy volunteers.
%K all-trans retinoic acid (ATRA)
%K LC-MS-MS
%K liquid-liquid extraction
%K bioequivalence
%U http://www.mdpi.com/1420-3049/19/1/1189