%0 Journal Article
%T New Natural Diterpene-Type Abietane from Tetradenia riparia Essential Oil with Cytotoxic and Antioxidant Activities
%A Zilda Cristiani Gazim
%A Felipe Rodrigues
%A Ana Carolina Louren£¿o Amorin
%A Cl¨¢udia Moraes de Rezende
%A Marina Sokovi£¿
%A Vele Te£¿evi£¿
%A Ivan Vu£¿kovi£¿
%A Gordana Krsti£¿
%A Lucia Elaine Ranieri Cortez
%A Nelson Barros Colauto
%A Giani Andrea Linde
%A Di¨®genes Apar¨ªcio Garcia Cortez
%J Molecules
%D 2014
%I MDPI AG
%R 10.3390/molecules19010514
%X Tetradenia riparia (Hochstetter) Codd belongs to the Lamiaceae family and it was introduced in Brazil as an exotic ornamental plant. A previous study showed its antimicrobial, acaricidal and analgesic activities. Two compounds were isolated from essential oil of T. riparia leaves and identified as 9¦Â,13¦Â-epoxy-7-abietene ( 1), a new one, and 6,7-dehydroroyleanone ( 2), already reported for another plant. The structure of these compounds was determined by spectroscopic analysis and by comparison with literature data. The cytotoxic activities of the essential oil and compounds 1 and 2 were determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, and by tumor cells MDA-MB-435 (human breast carcinoma), HCT-8 (human colon), SF-295 (human nervous system) and HL-60 (human promyelocytic leukemia). The essential oil and compound 1 showed high cytotoxic potential of the cell lines SF-295 (78.06% and 94.80%, respectively), HCT-8 (85.00% and 86.54%, respectively) and MDA-MB-435 (59.48% and 45.43%, respectively). Compound 2 had no cytotoxic activity. The antioxidant activity was determined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ¦Â-carotene-linoleic acid system and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The inhibitory concentration (IC 50 in ¦Ìg mL £¿1) for essential oil and compound 2 was, respectively 15.63 and 0.01 for DPPH; 130.1 and 109.6 for ¦Â-carotene-linoleic acid and 1524 and 1024 for ABTS. Compound 1 had no antioxidant activity. By fractioning the oil, it was possible to identify two unpublished compounds: 1 with high cytotoxic potential and 2 with high antioxidant potential.
%K Tetradenia riparia
%K essential oil
%K abietane diterpenes
%K 9¦Â
%K 13¦Â-epoxy-7-abietene
%K 6
%K 7-dehydroroyleanone
%K cytotoxicity activity
%K antioxidant activity
%U http://www.mdpi.com/1420-3049/19/1/514