%0 Journal Article
%T A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling
%A Wen-Li Hsu
%A Siou-Jin Chiu
%A Yao-Ting Tsai
%A Che-Mai Chang
%A Jaw-Yan Wang
%A Eric Terry Wang
%A Ming-Feng Hou
%A Chiung-Yao Huang
%A Jyh-Horng Sheu
%A Wei-Chiao Chang
%J Molecules
%D 2013
%I MDPI AG
%R 10.3390/molecules18067023
%X Epidermal growth factor receptor (EGFR) is overexpressed in many types of cancer cells. EGFR-mediated signaling involves inflammatory gene expression including cyclooxygenase (COX)-2 and interleukin (IL)-8, and is associated with cancer pathogenesis. In a search of phytochemicals with anti-inflammatory activity, the COX-2 and IL-8 inhibitory activities of some marine compounds were examined. After screening these compounds 11-episinulariolide acetate ( 1) from soft coral exhibited the most potent activity. Reverse-transcription PCR; western blotting; ELISA and luciferase assays were used to test the effect of compound 1 on EGF-stimulated expressions of COX-2 and IL-8 in A431 human epidermoid carcinoma cells. After exposure to 10 ¦ÌM of compound 1, expression levels of COX-2 and IL-8 were reduced. In addition; intracellular Ca 2+ increase and Ca 2+-dependent transcription factor activation were blocked by compound 1. Thus, compound 1 can potentially serve as a lead compound for targeting Ca 2+ signaling-dependent inflammatory diseases.
%K marine compound
%K EGF
%K COX-2
%K IL-8
%K calcium
%U http://www.mdpi.com/1420-3049/18/6/7023