%0 Journal Article
%T Anti-Inflammatory Activity of Different Agave Plants and the Compound Cantalasaponin-1
%A Nayeli Monterrosas-Brisson
%A Martha L. Arenas Ocampo
%A Enrique Jim¨¦nez-Ferrer
%A Antonio R. Jim¨¦nez-Aparicio
%A Alejandro Zamilpa
%A Manases Gonzalez-Cortazar
%A Jaime Tortoriello
%A Maribel Herrera-Ruiz
%J Molecules
%D 2013
%I MDPI AG
%R 10.3390/molecules18078136
%X Species of the agave genus, such as Agave tequilana, Agave angustifolia and Agave americana are used in Mexican traditional medicine to treat inflammation-associated conditions. These plants¡¯ leaves contain saponin compounds which show anti-inflammatory properties in different models. The goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema induced in mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) technique. A dose of 6 mg/ear of acetone extract from the three agave species induced anti-inflammatory effects, however, the one from A. americana proved to be the most active. Different fractions of this species showed biological activity. Finally the F5 fraction at 2.0 mg/ear induced an inhibition of 85.6%. We identified one compound in this fraction as (25 R)-5¦Á-spirostan-3¦Â,6¦Á,23¦Á-triol-3,6-di- O-¦Â-D-glucopyranoside (cantalasaponin-1) through 1H- and 13C-NMR spectral analysis and two dimensional experiments like DEPT NMR, COSY, HSQC and HMBC. This steroidal glycoside showed a dose dependent effect of up to 90% of ear edema inhibition at the highest dose of 1.5 mg/ear.
%K Agavaceae
%K A. angustifolia Haw
%K A. tequilana Weber
%K A. americana
%K cantalasaponin-1
%K inflammation
%U http://www.mdpi.com/1420-3049/18/7/8136