%0 Journal Article
%T Salicortin-Derivatives from Salix pseudo-lasiogyne Twigs Inhibit Adipogenesis in 3T3-L1 Cells via Modulation of C/EBP¦Á and SREBP1c Dependent Pathway
%A Mina Lee
%A Sang Hoon Lee
%A Jimmy Kang
%A Heejung Yang
%A Eun Ju Jeong
%A Hong Pyo Kim
%A Young Choong Kim
%A Sang Hyun Sung
%J Molecules
%D 2013
%I MDPI AG
%R 10.3390/molecules180910484
%X Obesity is reported to be associated with excessive growth of adipocyte mass tissue as a result of increases in the number and size of adipocytes differentiated from preadipocytes. To search for anti-adipogenic phytochemicals, we screened for inhibitory activities of various plant sources on adipocyte differentiation in 3T3-L1 preadipocytes. Among the sources, a methanolic extract of Salix pseudo-lasiogyne twigs (Salicaceae) reduced lipid accumulation in a concentration-dependent manner. During our search for anti-adipogenic constituents from S. pseudo-lasiogyne, five salicortin derivatives isolated from an EtOAc fraction of this plant and bearing 1-hydroxy-6-oxo-2-cyclohexene-carboxylate moieties, namely 2¡ä,6¡ä- O-acetylsalicortin ( 1), 2¡ä- O-acetylsalicortin ( 2), 3¡ä- O-acetylsalicortin ( 3), 6¡ä- O-acetylsalicortin ( 4), and salicortin ( 5), were found to significantly inhibit adipocyte differentiation in 3T3-L1 cells. In particular, 2¡ä,6¡ä- O-acetylsalicortin ( 1) had the most potent inhibitory activity on adipocyte differentiation, with an IC 50 value of 11.6 ¦ÌM, and it significantly down-regulated the expressions of CCAAT/enhancer binding protein ¦Á (C/EBP¦Á) and sterol regulatory element binding protein 1 (SREBP1c). Furthermore, 2¡ä,6¡ä- O-acetylsalicortin ( 1) suppressed mRNA expression levels of C/EBP¦Â during the early stage of adipocyte differentiation and stearoyl coenzyme A desaturase 1 (SCD-1), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS) expression, target genes of SREBP1c. In the present study, we demonstrate that the anti-adipogenesis mechanism of 2¡ä,6¡ä- O-acetylsalicortin ( 1) may be mediated via down-regulation of C/EBP¦Á and SREBP1c dependent pathways. Through their anti-adipogenic activity, salicortin derivatives may be potential novel therapeutic agents against obesity.
%K Salix pseudo-lasiogyne
%K Salicaceae
%K 3T3-L1
%K adipogenesis
%K adipocyte differentiation
%K C/EBP¦Á
%K SREBP1c
%K obesity
%U http://www.mdpi.com/1420-3049/18/9/10484