%0 Journal Article
%T Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
%A Amin Abbaszadeh Banaeiyan
%A Doryaneh Ahmadpour
%A Caroline Beck Adiels
%A Mattias Goks£¿r
%J Micromachines
%D 2013
%I MDPI AG
%R 10.3390/mi4040414
%X The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 ¦Ìm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min £¿1, moderate = 50 nL min £¿1, and high = 100 nL min £¿1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.
%K microfluidics
%K single cell
%K high-throughput
%K hydrodynamic trapping
%K yeast
%K arsenite
%K PDMS
%K fluorescence microscopy
%U http://www.mdpi.com/2072-666X/4/4/414