%0 Journal Article
%T The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part I (Migration)
%A Kathryn Dorst
%A Derek Rammelkamp
%A Michael Hadjiargyrou
%A Dilip Gersappe
%A Yizhi Meng
%J Materials
%D 2013
%I MDPI AG
%R 10.3390/ma6125517
%X Initial cell-surface interactions are guided by the material properties of substrate topography. To examine if these interactions are also modulated by the presence of zinc, we seeded murine pre-osteoblasts (MC3T3-E1, subclone 4) on micropatterned polydimethylsiloxane (PDMS) containing wide (20 ¦Ìm width, 30 ¦Ìm pitch, 2 ¦Ìm height) or narrow (2 ¦Ìm width, 10 ¦Ìm pitch, 2 ¦Ìm height) ridges, with flat PDMS and tissue culture polystyrene (TC) as controls. Zinc concentration was adjusted to mimic deficient (0.23 ¦ÌM), serum-level (3.6 ¦ÌM), and zinc-rich (50 ¦ÌM) conditions. Significant differences were observed in regard to cell morphology, motility, and contact guidance. We found that cells exhibited distinct anisotropic migration on the wide PDMS patterns under either zinc-deprived (0.23 ¦ÌM) or serum-level zinc conditions (3.6 ¦ÌM). However, this effect was absent in a zinc-rich environment (50 ¦ÌM). These results suggest that the contact guidance of pre-osteoblasts may be partly influenced by trace metals in the microenvironment of the extracellular matrix.
%K zinc
%K osteoblast
%K polydimethylsiloxane
%K micropatterns
%K contact guidance
%U http://www.mdpi.com/1996-1944/6/12/5517