%0 Journal Article
%T Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
%A Juan Peng
%A Shanshan Song
%A Liguang Xu
%A Wei Ma
%A Liqiang Liu
%A Hua Kuang
%A Chuanlai Xu
%J International Journal of Environmental Research and Public Health
%D 2013
%I MDPI AG
%R 10.3390/ijerph10072897
%X We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.
%K peanut allergen
%K Ara h 1
%K monoclonal antibody
%K sandwich ELISA
%U http://www.mdpi.com/1660-4601/10/7/2897