%0 Journal Article
%T Horizontal Transmission of Metarhizium anisopliae in Fruit Flies and Effect of Fungal Infection on Egg Laying and Fertility
%A Susan Dimbi
%A Nguya K. Maniania
%A Sunday Ekesi
%J Insects
%D 2013
%I MDPI AG
%R 10.3390/insects4020206
%X Fly-to-fly transmission of conidia of the entomopathogenic fungus Metarhizium anisopliae and the effect of fungal infection on the reproductive potential of females surviving infection were investigated in three fruit fly species, Ceratitis cosyra, C. fasciventris, and C. capitata. The number of conidia picked up by a single fruit fly was determined in C. cosyra. The initial uptake (Day 0) of conidia by a single fly was approx. 1.1 ℅ 10 6 conidia after exposure to the treated substrate. However, the number of conidia dropped from 7.2 ℅ 10 5 to 4.1 ℅ 10 5 conidia after 2 and 8 h post-exposure, respectively. The number of conidia picked up by a single fungus-treated fly (※donor§) varied between 3.8 ℅ 10 5 and 1.0 ℅ 10 6 in the three fruit fly species, resulting in 100% mortality 5每6 days post-exposure. When fungus-free flies of both sexes (※recipient§ flies) were allowed to mate with ※donor§ flies, the number of conidia picked up by a single fly varied between 1.0 ℅ 10 5 and 2.5 ℅ 10 5, resulting in a mortality of 83每100% in C. capitata, 72每85% in C. cosyra and 71每93% in C. fasciventris 10每15 days post-inoculation . There was an effect of fungal infection on female egg laying in the three species of fruit flies as control flies laid more eggs than fungus-treated females. The percentage reduction in fecundity in flies infected with M. anisopliae was 82, 73 and 37% in C. capitata, C. fasciventris and C. cosyra, respectively. The results are discussed with regard to application in autodissemination techniques.
%K Ceratitis capitata
%K C. fasciventris
%K C. cosyra
%K fruit fly
%K Metarhizium anisopliae
%K horizontal transmission
%K fungal infection
%K egg laying
%K fertility
%U http://www.mdpi.com/2075-4450/4/2/206