%0 Journal Article
%T Gut Transcription in Helicoverpa zea is Dynamically Altered in Response to Baculovirus Infection
%A Jeffrey E. Noland
%A Jonathan E. Breitenbach
%A Holly J. R. Popham
%A Sue M. Hum-Musser
%A Heiko Vogel
%A Richard O. Musser
%J Insects
%D 2013
%I MDPI AG
%R 10.3390/insects4030506
%X The Helicoverpa zea transcriptome was analyzed 24 h after H. zea larvae fed on artificial diet laced with Helicoverpa zea single nucleopolyhedrovirus (HzSNPV). Significant differential regulation of 1,139 putative genes ( p < 0.05 T-test with Benjamini and Hochberg False Discovery Rate) was detected in the gut epithelial tissue; where 63% of these genes were down-regulated and 37% of genes were up-regulated compared to the mock-infected control. Genes that play important roles in digestive physiology were noted as being generally down-regulated. Among these were aminopeptidases, trypsin-like serine proteases, lipases, esterases and serine proteases. Genes related to the immune response reacted in a complex nature having peptidoglycan binding and viral antigen recognition proteins and antiviral pathway systems down-regulated, whereas antimicrobial peptides and prophenoloxidase were up-regulated. In general, detoxification genes, specifically cytochrome P450 and glutathione S-transferase were down-regulated as a result of infection. This report offers the first comparative transcriptomic study of H. zea compared to HzSNPV infected H. zea and provides further groundwork that will lead to a larger understanding of transcriptional perturbations associated with viral infection and the host response to the viral insult in what is likely the most heavily infected tissue in the insect.
%K Helicoverpa zea
%K HzSNPV
%K gut epithelium
%K microarray
%U http://www.mdpi.com/2075-4450/4/3/506