%0 Journal Article
%T A Versatile Tool for Stable Inhibition of microRNA Activity
%A Paride Pelucchi
%A Valeria Tria
%A Valentina Martino
%A Davood Sabour
%A Giovanni Bertalot
%A Stefano Molgora
%A Mira Palizban
%A Martin G£¿tte
%A Ileana Zucchi
%A Rolland A. Reinbold
%J Biology
%D 2013
%I MDPI AG
%R 10.3390/biology2030861
%X MicroRNAs (miRNAs) are a class of small RNAs (18¨C22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.
%K miRNA
%K lentivirus
%K miR-inhibitor
%U http://www.mdpi.com/2079-7737/2/3/861