%0 Journal Article
%T Molecular Characterization of Begomoviruses and DNA Satellites Associated with a New Host Spanish Flag (Lantana camara) in India
%A Avinash Marwal
%A Anurag Kumar Sahu
%A Rajarshi Kumar Gaur
%J ISRN Virology
%D 2013
%R 10.5402/2013/915703
%X In the year 2010 yellowing of leaf vein disease was observed on Spanish Flag (Lantana camara) in Sirsa, Haryana province, India. There was no earlier report of association of begomovirus and DNA satellites with Lantana camara. Therefore, molecular characterization and understanding of the genomic analysis of begomovirus infecting Lantana camara is imperative for the pathogen diagnosis and disease management. This is the first report and molecular characterization of a begomovirus associated with its two satellites infecting a new host Lantana camara in India. 1. Introduction Geminiviridae family has been classified into four genera: Mastrevirus, Curtovirus, Topocuvirus, and Begomovirus [1]. Begomovirus is the largest genus of this family. The vector is the white fly (Bemisia tabaci) that causes begomoviral infections in ornamentals, crops, and weeds and it is prevalent in the tropical and subtropical regions of the world [2]. Begomovirus is the only genus of the Geminiviridae which has bipartite genome with virus genes resident on two different circular ssDNA molecules (DNA-A, DNA-B). The mainstream of begomoviruses are bipartite nature; however, few monopartite begomoviruses have been identified that are associated with DNA satellites (alphasatellites and betasatellites). Betasatellites induces disease symptoms in the hosts from which they were isolated, whereas alphasatellites play no role in symptoms development [3]; therefore, increasing knowledge about its epidemiology, sequence diversity, and biodiversity is highly important in order to implement preventative strategies. Spanish Flag (Lantana camara) was introduced in Europe from Central America as an ornamental plant and now is considered as one of the highest impacting invasive species throughout the globe. In India it is cultivated as an ornamental plant used for lining the boundaries of gardens as a shrub for its aesthetic beauty of small variety of attractive flowers. In the present report, we identified a begomovirus and its DNA satellites associated with Lantana camara through molecular techniques followed by its phylogenetic analysis. 2. Material and Methods During the survey for begomovirus infection 20¨C30% yellow vein disease was observed on Lantana camara in the nursery garden of Sirsa, Haryana province, India. To investigate the potential begomoviral infection, total DNA was extracted from leaves of 32 infected plants using the cetyl trimethyl ammonium bromide (CTAB) method [4]. To confirm the presence of begomovirus in infected samples PCR was performed using a pair of degenerate
%U http://www.hindawi.com/journals/isrn.virology/2013/915703/