%0 Journal Article
%T Effect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm
%A Lidia Gil
%A Iv¨˘n Galindo-Cardiel
%A C. Malo
%A N. Gonz¨˘lez
%A C. ¨˘lvarez
%J ISRN Veterinary Science
%D 2013
%R 10.1155/2013/280143
%X Cholesterol and Equex-STM are frequently added to different commercial and experimental extenders improving postthawing sperm quality. Doses of 125¨C150£żmM of cholesterol from pig liver and 0.5¨C0.7% of Equex-STM were evaluated in a standard eggyolk extender (Martin et al., 1979). Six ejaculates per stallion from six pure Spanish stallions (6¨C8 years old) were collected in Martin's extender (B) and different mixtures of 125£żmM-0.5% (I), 125£żmM-0.7% (II), 150£żmM-0.5% (III), and 150£żmM-0.7% (IV) were added to original Martin's extender. Samples were frozen in 0.5£żmL straws ( spermatozoa) and thawed (21£żs., water bath). After thawing the following parameters were evaluated: viability (V), motility (computer assisted sperm analysis, CASA; % nonprogressive NP; % progressive MP), hipoosmotic swelling test (HOST), acrosome integrity (A), fluorescence test (FL), and resistance test (RT). Sperm quality was significantly affected by stallion (in the parameters V, VI, NP, MP, HOST, A, FL, and RT), extraction (VI, NP, MP, HOST, A, and FL), and the different combinations of Equex-STM-cholesterol (FL). We concluded that 0.5% of Equex-STM mixed with 125 mM of cholesterol has obtained better sperm quality results than those of original Martin's extender, showing a simple and economic improvement of this home-made practical seminal extender. 1. Introduction Artificial insemination remains as one of the most important assisted reproductive technologies. It is a simple, economical and successful method for animal reproduction [1]. Freezing/thawing sperm techniques are a widely used method to manage equine breeds and maintain genetic stallion pool [2]. Different sperm cryopreserving methods have been developed for a lot of species, such as Sea Urchin (Evechinus chloroticus) [3], Carp (Cyprinus carpio) [4], Pacific oyster (Crassostrea gigas) [5], Red Deer (Cervus elaphus) [6], Human (Homo sapiens sapiens) [7], and practically all domestic and farmer species (reviewed by [8, 9]). Seminal cryopreservation techniques should be performed taking into account physiological individual stallion-depending sperm variation and the specific cooling, freezing, and thawing rates, such as the necessary equipment [10¨C14]. Frozen/thawed sperm protocols have been described as an improving reproductive tool related to a better breed management and reproductive potential stallion optimization due to its ability to preserve fertile inseminating doses [15, 16]. Cryopreservation extends the availability of sperm for fertilization; however, the fertilizing potential of the frozen-thawed sperm is
%U http://www.hindawi.com/journals/isrn.veterinary.science/2013/280143/