%0 Journal Article
%T Ixora coccinea Enhances Cutaneous Wound Healing by Upregulating the Expression of Collagen and Basic Fibroblast Growth Factor
%A Aadesh Upadhyay
%A Pronobesh Chattopadhyay
%A Danswrang Goyary
%A Papiya Mitra Mazumder
%A Vijay Veer
%J ISRN Pharmacology
%D 2014
%R 10.1155/2014/751824
%X Background. Ixora coccinea L. (Rubiaceae) has been documented for traditional use in hypertension, menstrual irregularities, sprain, chronic ulcer, and skin diseases. In the present study, I. coccinea was subjected to in vitro and in vivo wound healing investigation. Methods. Petroleum ether, chloroform, methanol, and water sequential I. coccinea leaves extracts were evaluated for in vitro antioxidant, antimicrobial, and fibroblast proliferation activities. The promising I. coccinea methanol extract (IxME) was screened for in vivo wound healing activity in Wistar rat using circular excision model. Wound contraction measurement, hydroxyproline quantification, and western blot for collagen type III (COL3A1), basic fibroblast growth factor (bFGF), and Smad-2, -3, -4, and -7 was performed with 7-day postoperative wound granulation tissue. Gentamicin sulfate (0.01% w/w) hydrogel was used as reference standard. Results. IxME showed the potent antimicrobial, antioxidant activities, with significant fibroblast proliferation inducing activity, as compared to all other extracts. In vivo study confirmed the wound healing accelerating potential of IxME, as evidenced by faster wound contraction, higher hydroxyproline content, and improved histopathology of granulation tissue. Western blot analysis revealed that the topical application of I. coccinea methanol extract stimulates the fibroblast growth factor and Smad mediated collagen production in wound tissue. 1. Background The World Health Organization estimated that 80% of the world¡¯s population still relies on plant-based medicines for their primary health care, and skin related disorders specially wounds, which is the most common reason for medical visits in the developing countries. Wounds and wound management therapy have a long history and in the different Ayurvedic classics of India like Charaka Samhita, Sushruta Samhita, and Ayurveda Siksha approximately 70% of the wound healing medicines are of plant origin. In the last few decades, traditional wound healing plants have received enough attention for scientific investigations [1¨C3], where pathophysiological process of wound healing and various related activities such as fibroblast growth stimulation and antioxidant and antimicrobial activities has been extensively studied and correlated to the rationale of the traditional plant medicines [1, 4, 5]. In thrust of finding for an effective wound healing herb,£¿ £¿Ixora coccinea L. (Rubiaceae) was selected for the in vitro and in vivo wound healing investigations. I. coccinea is a small-medium evergreen shrub,
%U http://www.hindawi.com/journals/isrn.pharmacology/2014/751824/