%0 Journal Article
%T Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
%A Let¨ªcia A. de C. Lara
%A Jo£¿o B. dos Santos
%A Juliana S. Veloso
%A Marcio Balestre
%A Filipe C. Alves
%A Monik E. Leite
%J ISRN Molecular Biology
%D 2014
%R 10.1155/2014/828102
%X The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F2 population of common bean evaluated by the straw test method. The DNA from 186 F2 plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F2:4 progenies and ten lines were evaluated, in a 14 ¡Á 14 triple lattice experimental design. The adjusted mean values of the F2:4 progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance¡ª13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection. 1. Introduction Phytopathogenic organisms are the main agents responsible for significant losses in the common bean (Phaseolus vulgaris L.) crop, often even making the crop unviable in certain regions. Among the diseases that have most limited yield, white mold (Sclerotinia sclerotiorum) stands out, especially in the irrigated common bean crop [1]. It is known that the best manner of controlling most common bean diseases is the use of cultivars with some level of genetic resistance. This measure is most recommended because it avoids or reduces the use of agricultural chemicals and is economically viable for the producer. However, for control of white mold, this measure is not efficient since cultivars with a satisfactory level of resistance that are adapted to Brazilian conditions are not available. Some lines and cultivars adapted to the conditions of west-central and southeast Brazil have partial resistance [2]. To evaluate common bean resistance to white mold, there are diverse methodologies that use artificial inoculation of the pathogen [3]. Among existing methods, greatest emphasis is placed on the straw test, described by Petzoldt and Dickson [4], due to its simplicity for evaluation of physiological resistance. The straw test assists in identification, characterization, and selection of genotypes resistant to white mold, and it is the most used method in breeding programs [5]. The complexity of resistance to white mold has led many researchers to analyses of QTLs for the purpose of
%U http://www.hindawi.com/journals/isrn.molecular.biology/2014/828102/