%0 Journal Article
%T In Vitro Cytotoxicity Evaluation of Four Vital Pulp Therapy Materials on L929 Fibroblasts
%A Aniket S. Wadajkar
%A Chul Ahn
%A Kytai T. Nguyen
%A Qiang Zhu
%A Takashi Komabayashi
%J ISRN Dentistry
%D 2014
%R 10.1155/2014/191068
%X The aim of this study was to evaluate cytotoxicity of direct pulp capping materials such as Dycal, Life, ProRoot MTA, and Super-Bond C&B on L929 fibroblasts. Freshly mixed or set materials were prepared and eluted by incubation with cell culture medium for working time period (fresh) or for 6 hours (set). The cells were exposed to media containing elutes for 24 hours, after which the cell survival was evaluated by MTS assays. In freshly mixed materials, average ¡À standard deviation % cell viabilities were 40.2 ¡À 14.0%, 43.7 ¡À 16.0%, 72.9 ¡À 12.7%, and 66.0 ¡À 13.6% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. There was no statistical difference in cell viabilities among material groups, whereas in set materials, the cell viabilities were 48.7 ¡À 14.8%, 37.2 ¡À 10.6%, 46.7 ¡À 15.2%, and 100 ¡À 21.9% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. Super-Bond C&B showed more cell viabilities than the other three material groups . The four vital pulp therapy materials had similar cytotoxicity when the materials were fresh. Super-Bond C&B was less cytotoxic than Dycal, Life, and ProRoot MTA after the materials were set, which suggests the use of SB-C&B in future in vivo clinical investigations. 1. Introduction Direct pulp capping is a treatment for exposed vital pulp, which uses a dental material to facilitate both the formation of reparative dentin from odontoblasts [1] and the maintenance of vital pulp [2]. These materials include calcium hydroxide or calcium hydroxide-based cements such as Dycal and Life [3, 4], mineral trioxide aggregate (MTA; ProRoot MTA) [5], and adhesive resins [6]. Selection of pulp capping materials is important to ensure dental pulp cell vitality. Historically, Hermann [7] discovered that calcium hydroxide is effective in repairing a pulp exposure site. Calcium hydroxide possesses antibacterial properties and promotes pulp tissue repair [8]. Thus, it is considered the ¡°gold standard¡± for direct pulp capping, and it has a long record of clinical success [9]. MTA was developed in 1993 and has been successfully used for pulp capping [10]. When MTA powder is mixed with water, its calcium oxide component reacts with the water and forms calcium hydroxide. Thus, MTA slowly releases calcium hydroxide while setting [11]. In addition, adhesive systems have also been suggested for use as direct pulp capping materials [12]. However, it has been believed that resin systems are inferior to calcium hydroxide-based cements, including MTA [13]. The exception would be a methyl methacrylate-/tributylborane- (MMA/TBB-)
%U http://www.hindawi.com/journals/isrn.dentistry/2014/191068/