%0 Journal Article
%T An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study
%A Lavanya Anumula
%A Swaroop Kumar
%A Venkata Suneel Kumar
%A Chandra Sekhar
%A Murali Krishna
%A Rama Mohan Pathapati
%A Prathi Venkata Sarath
%A Yamini Vadaganadam
%A Rakesh Kumar Manne
%A Srinath Mudlapudi
%J ISRN Dentistry
%D 2012
%R 10.5402/2012/989781
%X Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based sealer was the most effective and urethane dimethacrylate resin-based sealer was the least effective against Enterococcus faecalis, whereas glass-ionomer-based and polydimethyl-siloxane-based sealers were effective only for a short period. Inhibition of the bacterial growth is related to the direct contact of the microorganism with the sealer. 1. Introduction Bacteria or their byproducts are considered to be the primary etiological agents of pulpal necrosis and periapical lesions [1]. The main objective of endodontic therapy is therefore to eliminate bacteria from the infected root canal [2]. The majority of the bacteria found in the root canal system may be eliminated by the biomechanical cleaning and shaping of the root canal space. Failure of the root canal treatment is the result of microorganisms persisting in the apical portion of the root canal system, even in well-treated teeth [3] due to the anatomical complexities of many root canals, such as dentinal tubules, ramifications, deltas, and fins which cannot be sufficiently cleaned, even after meticulous mechanical procedures. Enterococcus faecalis is a recalcitrant candidate among the many causative agents of failed endodontic treatment [4]. 38% of the failed root canal systems were contaminated with Enterococcus faecalis [5]. Chronic failure of an endodontically treated tooth is due to ability of E. faecalis to bind to the collagen of the dentinal tubule and remain viable within the tubules [6]. These microorganisms have the ability to grow even in a low-nutrient environment and can survive in the root canals as a
%U http://www.hindawi.com/journals/isrn.dentistry/2012/989781/