%0 Journal Article
%T Occurrence of Biosurfactant Producing Bacillus spp. in Diverse Habitats
%A Sanket J. Joshi
%A Harish Suthar
%A Amit Kumar Yadav
%A Krushi Hingurao
%A Anuradha Nerurkar
%J ISRN Biotechnology
%D 2013
%R 10.5402/2013/652340
%X Diversity among biosurfactant producing Bacillus spp. from diverse habitats was studied among 77 isolates. Cluster analysis based on phenotypic characteristics using unweighted pair-group method with arithmetic averages (UPGMAs) method was performed. Bacillus isolates possessing high surface tension activity and five reference strains were subjected to amplified 16S rDNA restriction analysis (ARDRA). A correlation between the phenotypic and genotypic characterization of Bacillus spp. is explored. Most of the oil reservoir isolates showing high surface activity clustered with B. licheniformis and B. subtilis, the hot water spring isolates clustered in two ingroups, while the petroleum contaminated soil isolates were randomly distributed in all the three ingroups. Present work revealed that diversity exists in distribution of Bacillus spp. from thermal and hydrocarbon containing habitats where majority of organisms belonged to B. licheniformis and B. subtilis group. Isolate B. licheniformis TT42 produced biosurfactant which reduced the surface tension of water from 72£¿mNm£¿1 to 28£¿mNm£¿1, and 0.05£¿mNm£¿1 interfacial tension against crude oil at 8 0 ¡ã C. This isolate clustered with B. subtilis and B. licheniformis group on the basis of ARDRA. These findings increase the possibility of exploiting the Bacillus spp. from different habitats and their possible use in oil recovery. 1. Introduction Bacillus spp. are main workhorses for biotechnological applications. Their products are in the GRAS list (generally regarded as safe) of US Food and Drug Administration (USFDA) and hence are regarded harmless [1¨C3]. They produce a variety of products namely. extracellular enzymes, biopolymers, biosurfactants, biopesticides, and so forth from renewable resources and are ecofriendly. Biosurfactants are biologically produced surface-active compounds which are versatile process chemicals, and those from Bacillus spp. additionally possess the property of functionality under extreme conditions of pH, temperature, and salinity [4, 5]. These compounds have property of lowering surface and interfacial tensions of liquids. Biosurfactant production has been reported under thermophilic condition using renewable resources and by using statistically optimized medium [6, 7]. One of the potential uses of biosurfactants is in oil industry with minimum purity specification so that whole-cell broth could be used. The lipopeptide biosurfactants produced by B. subtilis and B. licheniformis are highly potent due to their surface tension reducing ability. B. licheniformis and B. subtilis have
%U http://www.hindawi.com/journals/isrn.biotechnology/2013/652340/