%0 Journal Article
%T Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense
%A Janki N. Thakker
%A Samiksha Patel
%A Pinakin C. Dhandhukia
%J ISRN Biotechnology
%D 2013
%R 10.5402/2013/601303
%X The aim of the present study was to scrutinize the response of banana (Grand Naine variety) plants when interacting with dead or live pathogen, Fusarium oxysporum f.sp. cubense, a causative agent of Panama disease. Response of plants was evaluated in terms of induction of defense-related marker enzyme activity, namely, peroxidase (POX), polyphenol oxidase (PPO), -1,3 glucanase, chitinase, and phenolics. Plant's interaction with live pathogen resulted in early induction of defense to restrain penetration as well as antimicrobial productions. However, pathogen overcame the defense of plant and caused disease. Interaction with dead pathogen resulted in escalating defense response in plants. Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen. Results obtained in the present study suggest that dead pathogen was able to mount defense response in plants and provide resistance to Panama disease upon subsequent exposure. Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease. 1. Introduction Musa acuminata (Banana) is one of the most important fruit crops of world as well as of India. Banana could be considered poor manĄ¯s apple, and it is the cheapest among all other fruits in the country. Fusarium wilt caused by Fusarium oxysporum f.sp. cubense (Foc) is the most destructive disease of banana [1]. The pathogen is soil-borne and remains viable up to several years and cause 20%¨C80% loss of banana. Several disease management strategies can be used such as crop rotation, burning infected plants or plant parts, and application of carbendazim [2]. Methods mentioned have limited success, and the application of synthetic fungicides may result in undesirable effects on the environment. An alternative to above strategies for managing fusarium wilt is the use of biological control. Biocontrol agent can be a beneficial organism (live or dead) or its part such as cell wall, protein, and oligosaccharides [3]. While using live organisms as a biocontrol agent, appropriate conditions for maintaining it should be strictly followed. Nevertheless, if part of the organism such as cell wall, protein, oligosaccharide, or attenuated/killed organism is used then strict conditions are not required. Plants, humans, and animals give instantaneous response to the pathogen or its part. Animals and humans produce antibodies against pathogen or vaccine, similarly plants response to pathogen attack by producing PR-proteins, defense-related enzymes [4],
%U http://www.hindawi.com/journals/isrn.biotechnology/2013/601303/