%0 Journal Article
%T Encapsulation of Phaseolus lunatus Protein Hydrolysate with Angiotensin-Converting Enzyme Inhibitory Activity
%A Jorge Carlos Ruiz Ruiz
%A Maira Rub¨ª Segura Campos
%A David Abram Betancur Ancona
%A Luis Antonio Chel Guerrero
%J ISRN Biotechnology
%D 2013
%R 10.5402/2013/341974
%X The objective of recent research has been to seek alternative therapeutic treatments; for this reason, the use of protein hydrolysates from diverse sources has been studied. A way to guarantee that these treatments reach the site of action is through protection with covers, such as microcapsules. Therefore, proteins from the legume Phaseolus lunatus were hydrolyzed and encapsulated with a blend of Delonix regia carboxymethylated gum/sodium alginate (50£¿:£¿50£¿w/w). Hydrolysis release conditions in a simulated gastrointestinal system were obtained using CaCl2 concentrations as the main factor, indicating that lower CaCl2 concentrations lead to an increased hydrolysis release. Beads obtained with 1.0£¿mM of CaCl2 exhibited a better hydrolysate release rate under intestinal simulated conditions and the proteins maintained an IC50 of 2.9£¿mg/mL. Capsules obtained with the blend of Delonix regia carboxymethylated gum/sodium alginate would be used for the controlled delivery of hydrolysates with potential use as nutraceutical or therapeutic agents. 1. Introduction Enzymatic hydrolysis of food proteins has produced various biologically active peptides with immunostimulating, opioid, antithrombotic, anticariogenic, and bactericidal or angiotensin-converting enzyme (ACE) inhibitory functions and has been the focus of recent research [1]. The renin angiotensin system plays an important role in blood pressure and in cardiac and vascular functions. Renin produces decapeptide angiotensin I from angiotensinogen. ACE catalyzes the formation of angiotensin II by cleaving the dipeptide from the C-terminal of angiotensin I in the vascular wall [2]. ACE inhibitors have been prescribed for hypertensive patients worldwide, and many clinical application data have demonstrated that ACE inhibitors significantly reduce the morbidity and mortality of patients with myocardial infarction or heart failure [3]. Thus, ACE inhibitors may induce skin rashes, angioneurotic edema, diarrhea, cough, and dizziness [4]. Because hypertensive patients often need life-long medical treatment, interest has been focused on the isolation and identification of ACE-inhibitors which may be obtained from new and varied sources like foods [5]. Legumes are widely consumed in south-eastern Mexico as a major dietary protein source in both human and animal diets. Phaseolus lunatus L. is distributed throughout Latin America, the southern United States, Canada, and many other regions worldwide. It is a reasonably drought-tolerant legume with reported yields as high as 1500£¿kg/ha and is known to be an efficient
%U http://www.hindawi.com/journals/isrn.biotechnology/2013/341974/